Development and Evaluation of the Biological Adjuvant Bivalent Vaccine for Preventing Newcastle Disease and Infectious Bursal Disease

نویسندگان

چکیده

Newcastle disease (ND) and infectious bursal (IBD) are both one of the most economically important diseases, which cause high infection rate mortality chickens restrict development poultry industry. Currently, vaccination is effective method to prevent ND IBD. In this study, biological adjuvant bivalent vaccine (rClone30-VP2L-chGM-CSF) was developed for preventing Furthermore, immune protective effects rClone30-VP2L-chGM-CSF were evaluated in specific-pathogen-free chickens. The experimental results indicated that immunized with significantly improved anti-IBDV anti-NDV antibody titer; level CD4+ T, CD8+ B+, MHC-II+, monocyte/macrophagocyte+ cells; concentrations IL-1β, IL-4, IL-17, IFN-α, IFN-β, IFN-γ; splenocyte proliferation rate. Anti-NDV titer reached theoretical protection value 4log2 at 7 days after immunization group; however, 14 rClone30-VP2L rClone30. These showed stimulated stronger response than those Chickens challenged virulent IBDV BC6/85, protected group. RNA not measured, there appeared be little apoptosis bursa Fabricius Therefore, a promising candidate against virus (IBDV) newcastle (NDV), it provides an idea developing other vaccines.

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ژورنال

عنوان ژورنال: Transboundary and Emerging Diseases

سال: 2023

ISSN: ['1865-1682', '1865-1674']

DOI: https://doi.org/10.1155/2023/5904280